ABCs of DNA aptamer and related assay development.
Aptamer-related technologies represent a revolutionary advancement in the capacity to rapidly develop new classes of targeting ligands. Structurally distinct RNA and DNA oligonucleotides, aptamers mimic small, protein-binding molecules and exhibit high binding affinity and selectivity. Although their molecular weight is relatively small-approximately one-tenth that of monoclonal antibodies.
Format: Electronic (PDF) Historical Data: 2014 - 2016; Number of Pages: 75; Report Summary; Table of Contents; Segmentation; Methodology; Request a Free Sample; Industry Insights. The global aptamers market size was valued at USD 723.6 million in 2016 and is projected to grow at a CAGR of 28.2% during the forecast period. Nowadays, this small multifunctional molecule is a widely studied.
Aptamers are short single-stranded oligonucleotides that are capable of binding various molecules with high affinity and specificity. When the technology of aptamer selection was developed almost a quarter of a century ago, a suggestion was immediately put forward that it might be a revolutionary start into solving many problems associated with diagnostics and the therapy of diseases.
Once the aptamer (nucleic acid) sequence is known, the aptamer (synthetic antibody) can be produced on the fly using an oligo synthesizer to meet ones immediate needs. Easy to label with reporters, enzymes, or fluorescent tags. Cost Benefit Comparison of Antibodies and Aptamers. PDF (97.3KB) Aptamers Against Virtually Any Target! Proteins.
Research Paper 505 DNA-enhanced peroxidase activity of a DNA aptamer-hemin complex Paola Travascio, Yingfu Li and Dipankar Sen Background: In vitro selection (SELEX) previously identified short single- stranded DNAs that specifically bound N-methylmesoporphyrin IX (NMM), a stable transition-state analogue for porphyrin-metallation reactions. Interestingly, iron(lll)-protoporphyrin (hemin) was.
Research Paper 35 Saccharide-RNA recognition in an aminoglycoside antibiotic-RNA aptamer complex Licong Jiang, Asif K Suri, Radovan Fiala and Dinshaw J Pate1 Background: Aminoglycoside antibiotics are known to target ribosomal, retroviral and catalytic RNAs with high affinity and specificity. Recently, in vitro selection experiments have identified RNA aptamers that bind to aminoglycoside.
Functional DNA aptamers as biotherapeutic molecules Erik William Orava Doctor of Philosophy, 2013 Graduate Department of Pharmaceutical Sciences University of Toronto Aptamers are single-stranded oligonucleotides, DNA or RNA, which can bind to a myriad of targets such as ions, peptides, proteins, drugs, organic and inorganic molecules.